Generator

Part:BBa_K749024

Designed by: Takuya Akutsu   Group: iGEM12_TMU-Tokyo   (2012-09-26)

consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida

Biosafety_Level1.png Safety.png

Consecutively produces PFDH (BBa_K749005), formaldehyde dehydrogenase from Pseudomonas putida.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 999
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 999
    Illegal NotI site found at 354
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 999
    Illegal NgoMIV site found at 95
    Illegal NgoMIV site found at 419
    Illegal NgoMIV site found at 644
    Illegal NgoMIV site found at 941
    Illegal AgeI site found at 272
    Illegal AgeI site found at 458
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 184


[edit]
Categories
Parameters
None